- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: THEM2 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human THEM2.
The immunogen is located within the last 50 amino acids of THEM2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: THEM2 antibody can be used for detection of THEM2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- POSITIVE CONTROL 1: Cat. No. 1211 - HepG2 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-201 - Human Liver Tissue Slide
- PURIFICATION: THEM2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: THEM2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: THEM2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: ACOT13
- ADDITIONAL NAMES: THEM2 Antibody: HT012, THEM2, PNAS-27, HT012, Acyl-coenzyme A thioesterase 13, Thioesterase superfamily member 2, Acyl-CoA thioesterase 13
- Protein Accession Number: NP_060943
- PROTEIN GI NUMBER: 8923812
- NCBI GENE ID NUMBER: 55856
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- THEM2 Antibody: THEM2, also known as ACOT13, belongs to the hotdog-fold superfamily and possesses thioesterase activity, with greater activity observed with longer chain acyl-CoAs such as myristoyl- and palmitoyl-CoA. THEM2 is highly expressed in several tissues such as heart, kidney, liver and brain and has been shown to be co-localized with beta-tubulin on microtubules. THEM2 interacts with StarD, a protein that plays a key role in fatty acid metabolism, and the addition of StarD to THEM2 increased its catalytic activity, suggesting that THEM2 plays a significant role in the metabolism of fatty acids. At least two isoforms of THEM2 are known to exist.
- 1: Cheng Z, Song F, Shan X, et al. Crystal structure of human thioesterase superfamily member 2. Biochem. Biophys. Res. Commun.2006; 349:172-7.
- 2: Wei J, Kang HW, and Cohen DE. Thioesterase superfamily member 2 (Them2)/acyl-CoA thioesterase 13 (Acot13): a homotetrameric hotdog fold thioesterase with selectivity for long-chain fatty acyl-CoAs. Biochem. J.2009; 421:311-22.
- 3: Cheng Z, Bao S, Shan X, et al. Human thioesterase superfamily member 2 (hTHEM2) is co-localized with beta-tubulin onto the microtubule. Biochem. Biophys. Res. Commun.2006; 350:850-3.
- 4: Scappa EF, Pocai A, Wu MK, et al. Regulation of energy substrate utilization and hepatic insulin sensitivity by phosphatidylcholine transfer protein/StarD. FASEB J.2008; 22:2579-90.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 5257
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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