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TGF-alpha Antibody [SPM542]
CATALOG NUMBER: 34-170- Clonality: Monoclonal
- Tested Applications: Flow, IF, IHC
- Host Species: Mouse
- Species Reactivity: Human, Rabbit, Zebrafish
- Conjugate: Unconjugated
- Size: 100 ug
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human, Rabbit, Zebrafish
- IMMUNOGEN: Amino acids 34-43 (PPVAAAVVSH) from the human protein were used as the immunogen for this anti-TGF alpha antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC
- APPLICATION NOTE: Flow Cytometry: 0.5-1 ug/million cells in 0.1ml
Immunofluorescence: 1-2 ug/ml
Immunohistochemistry (FFPE): 2-4 ug/ml for 30 min at RT (1)
The optimal dilution of the anti-TGF-alpha antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Properties
- PURIFICATION: Protein G affinity chromatography
- CLONALITY: Monoclonal
- ISOTYPE: IgM, kappa
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: TGFA
- ADDITIONAL NAMES: TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
- NCBI GENE ID NUMBER: 7039
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. TGFa is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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$35 Dry Ice Shipment fee may be required on some items.
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