- Trial Size
TET1 Antibody
CATALOG NUMBER: 7733- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (94%), Pig: (88%)
- IMMUNOGEN: Anti-TET1 antibody (7733) was raised against an 18 amino acid peptide near the carboxy terminus of human TET1.
The immunogen is located within amino acids 2030 - 2080 of TET1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1 μg/mL; IHC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot and Immunohistochemistry in human, mouse and rat samples; Immunocytochemistry in human samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - SPECIFICITY: TET1 antibody is human, mouse and rat reactive. This antibody is predicted to not cross-react with TET2 and TET3.
- POSITIVE CONTROL 1: Cat. No. 1405 – Mouse Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 11-701 - Human Testis Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 235 kDa
Observed: 235 kDa
Advanced Validation
- ISOFORMS: Human TET1 has one isoform (2136aa, 235kD). Mouse TET1 has one isoform (2007aa, 219kD) and Rat TET1 also has one isoform (2033aa, 223kD). 7733 can detect human, mouse and rat.
Properties
- PURIFICATION: TET1 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TET1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TET1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: TET1
- ADDITIONAL NAMES: TET1 Antibody: LCX, CXXC6, bA119F7.1, KIAA1676, LCX, Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6
- Protein Accession Number: NP_085128
- PROTEIN GI NUMBER: 156139122
- NCBI GENE ID NUMBER: 80312
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TET1, a member of the ten-eleven-translocation (TET) family of genes, was initially discovered as a fusion partner of MLL in acute myeloid leukemias containing the t(10;11)(q22;q23) (1). It is a methylcytosine dioxygenase that catalyzes the conversion of methylcytosine to 5-hydroxymethylcytosine (2). TET1 expression is regulated during inflammation in both THP-1 and primary dendritic cells, and is a negative transcriptional regulator of IL-1beta following and inflammatory stimulus (3). Recent evidence has shown that TET1 is part of a signaling pathway that regulates breast cancer growth and metastasis (4).
- 1: Lorsbach, et al. Leukemia 2003; 17:637-41.
- 2: Tahilani , et al. Science 2009; 324:930-5.
- 3: Neves-Costa, et al. Immunol. 2013; 54:264-70.
- 4: Sun, et al. Sci. USA 2013; 110:9920-5.
Citations
- 1: McLaughlin et al. Characterizing Requirements for Small Ubiquitin-like Modifier (SUMO) Modification and Binding on Base Excision Repair Activity of Thymine-DNA Glycosylase in Vivo. J Biol Chem. 2016;291(17):9014-24PMID: 26917720
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
![HIV-1 p24 Antibody [10A2] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-10A2_WB_PM-7371.gif){kind=small}
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
![HIV-1 p24 Antibody [9D2F6] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-9D2F6_WB_PM-7369.gif){kind=small}
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
![HIV-1 p24 Antibody [8D7E11] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-8D7E11_WB_PM-7367.gif){kind=small}
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin