- Trial Size
TEM7 Antibody
CATALOG NUMBER: 4339- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: TEM7 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human TEM7.
The immunogen is located within the last 50 amino acids of TEM7. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: TEM7 antibody can be used for detection of TEM7 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1304 - Human Liver Tissue Lysate
Properties
- PURIFICATION: TEM7 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TEM7 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TEM7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PLXDC1
- ADDITIONAL NAMES: TEM7 Antibody: TEM3, TEM7, TEM3, Plexin domain-containing protein 1, Tumor endothelial marker 3
- Protein Accession Number: AAG00869
- PROTEIN GI NUMBER: 14017379
- NCBI GENE ID NUMBER: 57125
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TEM7 Antibody: Tumor endothelial marker 7 (TEM7) was identified through serial analysis of gene expression on endothelial cells isolated from human normal and malignant colorectal tissues. Further experiments verified TEM7 was highly expressed in the endothelium of numerous other cancer types including breast, lung and brain tumors. At least four isoforms of TEM7 are known to exist; these include intracellular, secreted, and membrane-bound forms. A homologous protein, TEM7R (also known as PLXDC2), acts as a binding partner to TEM7 and is also abundantly expressed in the endothelium of malignant colorectal cancer but is absent or rare in normal colon mucosa. High expression of TEM7 is associated with metastasis and poor survival of patients with osteogenic sarcoma.
- 1: St. Croix B, Rago C, Velculescu V, et al. Genes expressed in human tumor endothelium. Science2000; 289:1197-202.
- 2: Nanda A, Buckhaults P, Seaman S, et al. Identification of a binding partner for the endothelial cell surface proteins TEM7 and TEM7R. Cancer Res.2004; 64:8507-11.
- 3: Carson-Weber EB, Watkins DN, Nanda A, et al. Cell surface tumor epithelial markers are conserved in mice and humans. Cancer Res.2001; 61:6649-55.
- 4: Fuchs B, Mahlum E, Halder C, et al. High expression of tumor endothelial marker 7 is associated with metastasis and poor survival of patients with osteogenic sarcoma. Gene2007; 399:137-43.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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