- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse (86%); Rat: (86%)
- IMMUNOGEN: Anti-TEM-1 antibody (4357) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1.
The immunogen is located within the last 50 amino acids of TEM-1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
- POSITIVE CONTROL 1: Cat. No. 1320 - Human Colon Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1223 - Caco-2 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 1219 – MCF-7 Cell Lysate
- POSITIVE CONTROL 4: Cat. No. 1220 – SK-N-SH Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 81kD and 46kD
Observed: 90 kD (post-modifications: 27 O-linked glycosylations) and 46kD
Independent Antibody Validation in Cell lines (Figure 2) shows similar TEM-1 expression profile in human and mouse cell lines detected by two independent anti-TEM-1 antibodies that recognize different epitopes, 4357 against C-terminus domain and 4359 against N-terminus domain. TEM-1 proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.
- ISOFORMS: Human TEM-1 has 2 isoforms, including isoform 1 (757aa, 81kD) and isoform 2 (433aa, 46kD). Mouse TEM-1 has one isoform (765aa, 82kD) and rat TEM-1 also has one isoform (764aa, 82kD). 4357 can detect all isoforms of human, mouse and rat.
- PURIFICATION: TEM1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TEM1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TEM1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: CD248
- ADDITIONAL NAMES: TEM1 Antibody: TEM1, CD164L1, CD248, Endosialin, Tumor endothelial marker 1
- Protein Accession Number: NP_065137
- PROTEIN GI NUMBER: 9966885
- NCBI GENE ID NUMBER: 57124
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- TEM1 Antibody: Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
- 1: Rettig et al. Proc. Natl. Acad. Sci. USA 1992; 89:10832-6.
- 2: Christian et al. J. Biol. Chem. 2001; 276:7408-14.
- 3: Nanda et al. Curr. Opin. Oncol. 2004; 16:44-9.
- 4: Nanda et al. Proc. Natl. Acad. Sci. USA 2006; 103:3351-6.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 4357
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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