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TCRab/Vb2 Antibody [TCR-3] (PE)
TCRab/Vb2 Antibody [TCR-3] (PE)

TCRab/Vb2 Antibody [TCR-3] (PE)

CATALOG NUMBER: 99-230
  • Clonality: Monoclonal
  • Tested Applications: EM, Flow, ICC, IP
  • Host Species: Mouse
  • Species Reactivity: Chicken, Guinea Fowl, Peacock
  • Conjugate: PE
  • Size: 0.1 mg

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Chicken, Guinea Fowl, Peacock
  • IMMUNOGEN: CD3+ TCR1- TCR2- Ia- chicken blood mononuclear cells
  • CONJUGATE: PE
  • TESTED APPLICATIONS: EM, Flow, ICC, IP
  • APPLICATION NOTE: TCRab/Vb2 Antibody [TCR-3] for use in flow cytometry assays.
  • SPECIFICITY: TCRalphabeta/Vbeta2

Properties

  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1
  • PHYSICAL STATE: Liquid
  • BUFFER: Supplied in PBS/NaN3 and a stabilizing agent
  • CONCENTRATION: 0.1 mg/mL
  • STORAGE CONDITIONS: Store vial at 2-8°C

Additional Info

  • ADDITIONAL NAMES: T3/TCR complex, TCR alpha/beta
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • The monoclonal antibody TCR-3 precipitates a CD3-associated heterodimer of Mr 88 kDa (two bands of Mr 48 kDa and 40 kDa upon reduction) on chicken peripheral blood T cells. Deglycosylation of the heterodimer yields two polypeptides of Mr 34 kDa and 31 kDa. In the chicken, two distinct subpopulations of alphabeta T cells appear in the thymus subsequent to the appearance of gammadelta T cells. These subpopulations, originally denoted as TCR-2 and TCR-3, arise sequentially in the thymus during ontogeny and are now known to represent two distinct Vbeta families, Vbeta1 and Vbeta2, respectively. The TCR-3 monoclonal antibody reacts with approximately 9% of thymocytes, 15-25% of blood mononuclear cells, and 13% of splenocytes young adult chickens. Two-color immunofluorescence has revealed that the TCR-3+ thymocytes include CD4+CD8-, CD4-CD8+, CD4+CD8+, and CD4-CD8- subpopulations. The TCR-3+ thymocytes can be separated into two subsets. One subset is characterized by relatively low levels of expression of the TCR-3/CD3 complex and most of the cells in this subset are CD4+CD8+. Cells in the other subset express TCR-3/CD3 in higher density and are either CD4+CD8- or CD4-CD8+, corresponding to the more mature medullary subset of thymocytes. The TCR-3+] cells in the blood and spleen express relatively high levels of the TCR-3/CD3 receptor complex and are “single positive” with CD4+CD8- cells being four times more frequent that the CD4-CD8+ cells (~ 80% CD4+ vs ~ 20% CD8+).

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 99-230

  • Size: 0.1 mg
  • List Price: $380.00
Datasheet
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