- Trial Size
TCF12 Antibody
CATALOG NUMBER: 5999- Clonality: Polyclonal
- Tested Applications: ELISA, ICC, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (95%)
- IMMUNOGEN: TCF12 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human TCF12.
The immunogen is located within amino acids 50 - 100 of TCF12. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, ICC, WB
- APPLICATION NOTE: TCF12 antibody can be used for detection of TCF12 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in human samples and Immunocytochemistry in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1201 - HeLa Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-001 - HeLa Cell Slide
Properties
- PURIFICATION: TCF12 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TCF12 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TCF12 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TCF12
- ADDITIONAL NAMES: TCF12 Antibody: HEB, CRS3, HTF4, bHLHb20, HsT17266, BHLHB20, HEB, Transcription factor 12, Class B basic helix-loop-helix protein 20, TCF-12
- Protein Accession Number: AAH50556
- PROTEIN GI NUMBER: 29792012
- NCBI GENE ID NUMBER: 6938
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TCF12 Antibody: TCF12, also known as HTF4, is a member of the basic helix-loop-helix (bHLH) E-protein family that recognizes the consensus binding site (E-box) CANNTG. TCF12 is expressed in many tissues, among them skeletal muscle, thymus, B- and T-cells, and may participate in regulating lineage-specific gene expression through the formation of heterodimers with other bHLH E-proteins. TCF12, in combination with E2A, is required to block thymocyte proliferation prior to pre-TCR expression and is critical for proper T cell differentiation. Recent reports have shown that TCF12 is also a critical factor required for the development of invariant natural killer T cells.
- 1: Zhang Y and Bina M. The nucelotide sequence of the human transcription factor HTF4a cDNA. DNA Seq.1992; 2:397-403.
- 2: Murre C, McCaw PS, Vaesin H, et al. Interactions between heterologous helix-loop-helix proteins generate complexes that bind specifically to a common DNA sequence. Cell1989; 58:537-44.
- 3: Wojciechowski J, Lai A, Kondo M, et al. E2A and HEB are required to block thymocyte proliferation prior to pre-TCR expression. J. Immunol.2007; 178:5717-26.
- 4: D'Cruz LM, Knell J, Fujimoto JK, et al. An essential role for the transcription factor HEB in thymocyte survival, Tcra rearrangement and the development of natural killer T cells. Nat. Immunol.2010; 11:240-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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