- Trial Size
TBX21 Antibody
CATALOG NUMBER: 7477- Clonality: Polyclonal
- Tested Applications: E, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: TBX21 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human TBX2.
The immunogen is located within the last 50 amino acids of TBX21 . - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, WB
- APPLICATION NOTE: TBX21 antibody can be used for detection of TBX21 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: TBX21 antibody is human and mouse reactive.
- POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-010 - HEK293 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 59 kDa
Observed: 55kDa
Properties
- PURIFICATION: TBX21 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TBX21 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TBX21 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: TBX21
- ADDITIONAL NAMES: TBX21 Antibody : TBET, T-PET, T-bet, TBLYM, TBET, T-box transcription factor TBX21, T-cell-specific T-box transcription factor T-bet, T-box protein 21
- Protein Accession Number: NP_037483
- PROTEIN GI NUMBER: 7019549
- NCBI GENE ID NUMBER: 30009
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TBX21 is a member of a phylogenetically conserved family of genes that share a common DNA-binding domain, the T-box (1,2). Members of this family include transcription factors involved in the regulation of developmental processes (2). Studies in mouse and humans show that TBX21 is a Th1 cell-specific transcription factor that controls the expression of the hallmark Th1 cytokine, interferon-gamma (1,3). Expression of the human ortholog also correlates with interferon-gamma expression in Th1 and natural killer cells, suggesting a TBX21 may play a role in initiating Th1 lineage development from naive Th precursor cells. (3).
- 1: Szabo SJ, Kim ST, Costa GL, et al. A novel transcription factor, T-bet, directs Th1 lineage commitment. Cell 2000; 100:655-69.
- 2: Naiche LA, Harrelson Z, Kelly RG, et al. T-box genes in vertebrate development. Annu. Rev. Genet. 2005; 39:219-39.
- 3: Hibbert L, Pflanz S, De Waal Malefyt R, et al. IL-27 and IFN-alpha signal via Stat1 and Stat3 and induce T-Bet and IL-12Rbeta2 in naive T cells. J. Interfeon Cytokine Res. 2003; 23:513-22.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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