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TBC1D4 Antibody
CATALOG NUMBER: 4415- Clonality: Polyclonal
- Tested Applications: ELISA, IHC-P
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (89%)
- IMMUNOGEN: TBC1D4 antibody was raised against an 19 amino acid peptide near the carboxy terminus of human TBC1D4 .
The immunogen is located within amino acids 1190 - 1240 of TBC1D4. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IHC-P
- APPLICATION NOTE: TBC1D4 antibody can be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 10-635 - Human Prostate Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 143 kDa
Observed: 140 kDa
Properties
- PURIFICATION: TBC1D4 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TBC1D4 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TBC1D4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: TBC1D4
- ADDITIONAL NAMES: TBC1D4 Antibody: AS160, AS160, KIAA0603, TBC1 domain family member 4, Akt substrate of 160 kDa
- Protein Accession Number: NP_055647
- PROTEIN GI NUMBER: 114688046
- NCBI GENE ID NUMBER: 9882
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TBC1D4 Antibody: TBC1D4, also known as the Akt substrate AS160, was initially identified as an Akt substrate containing a GTPase-activating domain that regulates GLUT4 trafficking, with activation following insulin stimulation. TBC1D4 truncations in humans is a major cause of dominant inherited insulin resistance. The loss of TBC1D4 results in the accumulation of GLUT4 in compartments that are primed for fusion in basal adipocytes.
- 1: Kane S, Sano H, Liu SCH, et al. Akt phosphorylates a novel adipocyte protein with a Rab GTPase-activating protein (GAP) domain. J. Biol. Chem. 2002; 277:22115-8.
- 2: Sano H, Kane S, Sano E, et al. Insulin-stimulated phosphorylation of a Rab GTPase-activating protein regulates GLUT4 translocation. J. Biol. Chem. 2003; 278:14599-602.
- 3: Dash S, Sano H, Rochford JJ, et al. A truncation mutation in TBC1D4 in a family with acanthosis nigricans and postprandial hyperinsulinemia. Proc. Natl. Acad. Sci. U.S.A. 2009; 106:9350–5.
- 4: Brewer PD, Romenskaia I, Kanow MA, et al. Loss of AS160 Akt substrate causes Glut4 protein to accumulate in compartments that are primed for fusion in basal adipocytes. J. Biol. Chem. 2011; 286:26287-97.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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