- Trial Size
TBC1D1 Antibody
CATALOG NUMBER: 4231- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (71%)
- IMMUNOGEN: TBC1D1 antibody was raised against a 22 amino acid synthetic peptide from near the carboxy terminus of human TBC1D1.
The immunogen is located within the last 50 amino acids of TBC1D1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: TBC1D1 antibody can be used for detection of TBC1D1 by Western blot at 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1210 – 293 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 128 kDa
Observed: 140 kDa
Properties
- PURIFICATION: TBC1D1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: TBC1D1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: TBC1D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: TBC1D1
- ADDITIONAL NAMES: TBC1D1 Antibody: TBC, TBC1, KIAA1108, TBC1 domain family member 1
- Protein Accession Number: NP_055988
- PROTEIN GI NUMBER: 50658061
- NCBI GENE ID NUMBER: 23216
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- TBC1D1 Antibody: TBC1D1 is the founding member of a family of proteins sharing a 180- to 200-amino acid TBC domain and presumed to have a role in regulating cell growth and differentiation. These proteins share significant homology with TRE2/USP6, yeast Bub2, and CDC16. TBC1D1 and TBC1D4 (AS160) have been demonstrated to be Rab GAPs (GTPase-activating proteins) that link upstream to Akt and phosphoinositide 3-kinase and downstream to Rabs involved in trafficking of GLUT4 vesicles. TBC1D1 regulates insulin-mediated GLUT4 translocation through its GAP activity, and is a likely Akt substrate. Mutations in the Tbc1d1 gene lead to some cases of severe human obesity.
- 1: White RA, Pasztor LM, Richardson PM, et al. The gene encoding TBC1D1 with homology to the tre-2/USP6 oncogene, Bub2, and cdc16 maps to mouse chromosome 5 and human chromosome 4. Cytogenet. Cell Genet.2000; 89:272-5.
- 2: Koumanov F and Holman GD. Thrifty Tbc1d1 and Tbc1d4 proteins link signalling and membrane trafficking pathways. Biochem. J.2007; 403:e9-11.
- 3: Roach WG, Chavez JA, Miinea CP, et al. Substrate specificity and effect on GLUT4 translocation of the Rab GTPase-activating protein Tbc1d1. Biochem J.2007; 403:353-8.
- 4: Stone S, Abkevich V, Russell DL, et al. TBC1D1 is a candidate for a severe obesity gene and evidence for a gene/gene interaction in obesity predisposition. Hum. Mol. Genet.2006; 15:2709-20.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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