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- SPECIES: Human, Mouse
- SOURCE SPECIES: E. coli
- RECOMBINANT PROTEIN SEQUENCE: The extracellular domain of mouse TRAIL (aa 99-291) is fused at the N-terminus to a His-tag and a linker peptide. The active multimeric conformation is stabilized by an inserted mutation allowing an additional CC-bridge.
- FUSION TAG: His Tag
- APPLICATION NOTE: This recombinant proteins is for research use only.
- BIOLOGICAL ACTIVITY: Induces apoptosis at concentrations of >10ng/ml as tested on human tumor cells. This effect can be efficiently blocked by recombinant mouse TRAIL receptors. This protein does not require a cross-linker (enhancer) for its potent biological activity.
Properties
- PURITY: >95% (SDS-PAGE).
Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza). - PHYSICAL STATE: Liquid
- BUFFER: In 20mM HEPES, pH 7.4, 300mM NaCl, 0.01% Tween 20, 1% sucrose, 1mM DTT.
- CONCENTRATION: 0.5mg/ml
- STORAGE CONDITIONS: Once thawed it is recommended to prepare appropriate aliquots and to store them at -80°C.
Additional Info
- NCBI OFFICIAL SYMBOL: Tnfsf10
- ADDITIONAL NAMES: TRAIL, Apo-2L, TNFSF10, CD253
- Protein Accession Number: P50592
- PROTEIN GI NUMBER: 1730016
- NCBI GENE ID NUMBER: 22035
Background
- TNF-related apoptosis-inducing ligand (TRAIL; Apo2L;CD253; TNFSF10) is a type II transmembrane protein of about 34kDa. Like most members of the tumor necrosis factor (TNF) superfamily of cytokines TRAIL can be cleaved at the cell surface by metalloproteases to form a soluble molecule. Active TRAIL forms trimers and specifically binds to five distinct receptors: TRAIL-R1 (DR4; Apo2;CD261; TNFRSF10A), TRAIL-R2 (DR5; KILLER; TRICK2A;TRICK2B; CD262; TNFRSF10B), TRAIL-R3 (DcR1;LIT; TRID; CD263; TNFRSF10C), TRAIL-R4 (DcR2; TRUNDD; CD264; TNFRSF10D), and osteoprotegerin (OPG; OCIF; TNFRSF11B). Trimerized TRAIL triggers apoptosis upon ligation of cell surface TRAIL-R1 and/or TRAIL-R2 by inducing the formation of the so-called multiprotein death-inducing signaling complex (DISC).
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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