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SUMO-2 Antibody [S2MT-1]SUMO-2 Antibody [S2MT-1]
SUMO-2 Antibody [S2MT-1]
SUMO-2 Antibody [S2MT-1]

SUMO-2 Antibody [S2MT-1]

CATALOG NUMBER: 33-903
  • Clonality: Monoclonal
  • Tested Applications: IF, IHC-P
  • Host Species: Mouse
  • Species Reactivity: Human, Rat
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human, Rat
  • IMMUNOGEN: Recombinant human protein was used as the immunogen for the SUMO-2 antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: IF, IHC-P
  • APPLICATION NOTE: Immunofluorescence: 0.5-1 ug/ml
    Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
    Prediluted format: incubate for 30 min at RT (2)
    Titering of the SUMO-2 antibody may be required for optimal performance.

    1. FFPE testing requires sections to be boiled in pH6 10mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: SUMO2
  • ADDITIONAL NAMES: SUMO2/3 Antibody: HSMT3, SMT3B, SUMO3, Smt3A, SMT3H2, SMT3A, Small ubiquitin-related modifier 2, HSMT3, SUMO-2
  • NCBI GENE ID NUMBER: 6613
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, 2 and 3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis and protein stability. The unconjugated SUMO-1, 2 and 3 proteins localize to the nuclear membrane, nuclear bodies and cytoplasm, respectively. SUMO-2 and 3 contribute to a greater percentage of protein modification than does SUMO-1 and unlike SUMO-1, they can form polymeric chains.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-903

  • Size: 100 ug
  • List Price: $555.00
Datasheet
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