- Trial Size
StrepII-tag Antibody
CATALOG NUMBER: 4337- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Tag
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Tag
- IMMUNOGEN: Anti-StrepII-tag antibody (4337) StrepII-tag antibody was raised against the amino acid sequence "asWSHPQFEKga".
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: WB: 0.25-1 μg/mL
Antibody validated: StrepII-tag antibody can be used for the detection of StrepII-tagged proteins
Advanced Validation
- VALIDATION:
Recombinant Protein Test (Figure 1&2): Anti-StrepII-tag antibodies (4337) detected StrepII-tagged recombinant protein at different concentrations
Properties
- PURIFICATION: StrepII-tag Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: StrepII-tag Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: StrepII-tag antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- ADDITIONAL NAMES: StrepII-tag Antibody:
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- StrepII-tag Antibody: Streptavidin is a tetrameric protein purified from Streptomyces avidinii. It has wide use in numerous molecular biological protocols dues to its strong affinity for biotin. The original Strep-tag is a nine amino acid peptide with high specificity and affinity towards streptavidin. The addition of this tag to the C-terminus of recombinant proteins allowed the simple purification of protein by use of affinity columns. It was re-engineered to allow it to also placed at the N-terminus of recombinant proteins.
- 1: Schmidt and SKerra. Protein Eng.1993;6:109-22.
- 2: Schmidt et al. J. Mol. Biol. 1996;255:753-6.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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