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sRANK Ligand Antibody
sRANK Ligand Antibody
sRANK Ligand Antibody
sRANK Ligand Antibody
sRANK Ligand Antibody
sRANK Ligand Antibody
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sRANK Ligand Antibody

CATALOG NUMBER: 3963
  • Clonality: Polyclonal
  • Tested Applications: E, IHC-P, WB
  • Host Species: Rabbit
  • Species Reactivity: Human, Mouse, Rat
  • Conjugate: Unconjugated
  • Size: 0.02 mg | 0.1 mg

Specifications

  • HOST SPECIES: Rabbit
  • SPECIES REACTIVITY: Human, Mouse, Rat
  • IMMUNOGEN: Anti-sRANK-L antibody (3963) was raised against a peptide corresponding to 14 amino acids near the center of human sRANK-L.
    The immunogen is located within amino acids 140-190 of sRANK-L.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: E, IHC-P, WB
  • APPLICATION NOTE: WB: 0.25-4 μg/mL; IHC: 5 μg/mL.
    Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human and rat samples. All other applications and species not yet tested.
  • POSITIVE CONTROL 1: Cat. No. 1464 - Rat Liver Tissue Lysate
  • POSITIVE CONTROL 2: Cat. No. 1304 - Human Liver Tissue Lysate
  • POSITIVE CONTROL 3: Cat. No. 10-201 - Human Liver Tissue Slide
  • POSITIVE CONTROL 4: Cat. No. 1404 - Mouse Liver Tissue Lysate
  • PREDICTED MOLECULAR WEIGHT: Predicted: 36kD
    Observed: 36 kD

Advanced Validation

  • VALIDATION:

    Recombinant Protein Test (Figure 3): Anti-sRANK-L antibodies (3963) detected human sRANK-L recombinant protein at different concentrations.

    Induced expression validation (Figure 6): sRANK-L expression detected by anit-sRANK-L antibodies (3963) was up-regulated after 18hr by extraction of  upper jaw molars in rat osteogenic cells.

  • ISOFORMS: Human sRANK-L has 3 isoforms, including isoform 1 (317aa, 35.5kD), isoform 2 (244aa, 27.7kD) and isoform 3 (270aa, 30.5kD). Mouse sRANK-L also has 3 isoforms, including isoform 1 (316aa, 35kD), isoform 2 (287aa, 32.3kD) and isoform 3 (199aa, 22.2kD). Rat sRANK-L has one isoform (318aa, 38.4kD). 3963 can detect human, mouse and rat.

Properties

  • PURIFICATION: sRANK Ligand Antibody is affinity chromatography purified via peptide column.
  • CLONALITY: Polyclonal
  • ISOTYPE: IgG
  • PHYSICAL STATE: Liquid
  • BUFFER: sRANK Ligand Antibody is supplied in PBS containing 0.02% sodium azide.
  • CONCENTRATION: 1 mg/mL
  • STORAGE CONDITIONS: sRANK Ligand antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

  • NCBI OFFICIAL SYMBOL: TNFSF11
  • ADDITIONAL NAMES: sRANK Ligand Antibody: ODF, OPGL, sOdf, CD254, OPTB2, RANKL, TRANCE, hRANKL2, Tumor necrosis factor ligand superfamily member 11, Osteoclast differentiation factor, ODF
  • Protein Accession Number: NP_003692
  • PROTEIN GI NUMBER: 4507595
  • NCBI GENE ID NUMBER: 8600
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background

  • sRANK Ligand Antibody: The receptor activator of NF-κB ligand (RANK-L) is a recently discovered member of the TNF-ligand family involved in the regulation of the T cell-dependent immune response, lymph node organogenesis and bone formation. RANK-L exists as both a normal, transmembrane form and a truncated, soluble form (sRANK-L), both of which can stimulate the receptor. Activation of T cells, such as by treatment with interleukin-7, induces RANK-L production and leads to an increase of osteoclast formation and bone loss. Finally, sRANK-L can activate the antiapoptotic kinase Akt through a signaling complex involving Src kinase and TRAF6, suggesting sRANK-L may also play a role in regulating apoptosis. This antibody will recognize both the soluble form and the uncleaved transmembrane form of RANK-L.
  • 1: Wong, et al. J. Biol. Chem. 1997; 272:25190-4.
  • 2: Kong et al. Nature 1999; 397:315-23.
  • 3: Weitzmann et al. Blood 2000; 96:1873-8.
  • 4: Bharti et al. J. Biol. Chem. 2004; 279:6065-76.

Citations

  • 1: Hassan et al. Coordination of early cellular reactions during activation of bone resorption in the rat mandible periosteum: An immunohistochemical study. Heliyon. 2017;3(10):e00430. PMID: PMID: 29226261.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 3963

  • Size: 0.02 mg | 0.1 mg
  • List Price: $89.00 – $395.00
Datasheet
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