- Trial Size
Spred3 Antibody
CATALOG NUMBER: 4851- Clonality: Polyclonal
- Tested Applications: ELISA, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Spred3 antibody was raised against a 16 amino acid synthetic peptide near the center of the human Spred3.
The immunogen is located within amino acids 140 - 190 of Spred3. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
- APPLICATION NOTE: Spred3 antibody can be used for detection of Spred3 by Western blot at 2 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: This Spred3 antibody is predicted to have no cross-reactivity to Spred1 or Spred2.
- POSITIVE CONTROL 1: Cat. No. 1403 - Mouse Brain Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-301 - Human Brain Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 45 kDa
Observed: 45 kDa
Properties
- PURIFICATION: Spred3 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Spred3 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Spred3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: SPRED3
- ADDITIONAL NAMES: Spred3 Antibody: Eve-3, spred-3, EVE-3, Sprouty-related, EVH1 domain-containing protein 3, Spred-3
- Protein Accession Number: NP_001035987
- PROTEIN GI NUMBER: 110225353
- NCBI GENE ID NUMBER: 399473
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Spred3 Antibody: Spred3 is a member of the Sprouty family, a group of proteins that act as negative regulators during development. Like Spred1 and Spred2, Spred3 contains and EVH1 domain in its amino terminus and a Sprouty-related cysteine-rich (SPR) domain in its C-terminus, but does not possess a functional c-kit binding domain (KBD). The Spred proteins have also been implicated in the negative feedback regulation of FGF signaling in embryogenesis and angiogenesis. Although Spred3 was initially reported to be expressed in brain, at least one report also indicates that Spred3 can be found in liver. At least two isoforms of Spred3 are known to exist.
- 1: Kato R, Nonami A, Taketomi T, et al. Molecular cloning of mammalian Spred-3 which suppresses tyrosine kinase-mediated Erk activation. Biochem. Biophys. Res. Commun.2003; 302:767-72.
- 2: Casci T, Vinos J, and Freeman M. Sprouty, an intracellular inhibitor of Ras signaling. Cell1999; 96:655-65.
- 3: Minowada G, Jarvis LA, Chi CL, et al. Vertebrate Sprouty genes are induced by FGF signaling and can cause chondrodysplasia when overexpressed. Development1999; 126:4465-75.
- 4: Lee SH, Schloss DJ, Jarvis L, et al. Inhibition of angiogenesis by a mouse sprouty protein. J. Biol. Chem.2001; 276:4128-33.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
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