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sIL-2Ra Antibody [SB-647]
CATALOG NUMBER: 38-230- Clonality: Monoclonal
- Tested Applications: ELISA, Neutralization Assay, WB
- Host Species: Mouse
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.5 mg
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: sIL-2Ra antibody is produced in mice using highly pure (>98%) recombinant human soluble IL-2 Receptor alpha as the immunizing antigen.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, Neutralization Assay, WB
- APPLICATION NOTE: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hsIL-2Rα (10.0 μg/mL), a concentration of 1.0-1.5 μg/mL of this antibody is required, in the presence of 0.5 ng/mL recombinant hIL-2.
ELISA:
In a sandwich ELISA (assuming 100 μL/well), a concentration of 4.0-8.0 μg/mL of this antibody will detect at least 100 pg/mL of recombinant human sIL-2Rα when used in conjunction with compatible secondary reagents.
Western Blot:
To detect hsIL-2Rα by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsIL-2Rα is 2.0-4.0 ng/lane, under reducing or non-reducing conditions
Properties
- PURIFICATION: This IgG2aK antibody was purified from cell culture by Protein G affinity chromatography.
- CLONALITY: Monoclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: sIL-2Ra lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.
Additional Info
- NCBI OFFICIAL SYMBOL: IL2RA
- ADDITIONAL NAMES: Interleukin-2 receptor subunit alpha, TAC antigen, IL-2 receptor subunit alpha, CD25, IL2R, TCGFR, IDDM10
- Protein Accession Number: P01589
- PROTEIN GI NUMBER: 124317
- NCBI GENE ID NUMBER: 3559
- USER NOTE: Centrifuge vial prior to opening.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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