Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: SF1 (phospho Ser82) antibody was raised against a peptide sequence around phosphorylation site of Serine 82 (S-P-S (p)-P-E) derived from Human SF1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- APPLICATION NOTE: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100
- SPECIFICITY: The antibody detects endogenous levels of SF1 only when phosphorylated at serine 82.
- PREDICTED MOLECULAR WEIGHT: 68 kDa
Properties
- PURIFICATION: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: Store antibody at -20°C for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: SF1
- ADDITIONAL NAMES: BBP, CW17, SF01, ZFM1, ZNF162
- Protein Accession Number: NP_004621.2
- PROTEIN GI NUMBER: 42544130
- NCBI GENE ID NUMBER: 7536
Background
- Necessary for the ATP-dependent first step of spliceosome assembly. Binds to the intron branch point sequence (BPS) 5'-UACUAAC-3' of the pre-mRNA. May act as transcription repressor.
- 1: Susan Y. Park, Development, May 2005; 132: 2415 - 2423.
- 2: Dujin Zhou, Mol. Endocrinol., Jul 2000; 14: 986.
- 3: Dagmar Wilhelm, Genes & Dev., Jul 2002; 16: 1839.
- 4: Philipp Gut,Development, Oct 2005; 132: 4611 - 4619.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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