- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (77%)
- IMMUNOGEN: SESTRIN2 antibody was raised against a 19 amino acid peptide near the center of human SESTRIN2.
The immunogen is located within amino acids 220 - 270 of SESTRIN2.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: SESTRIN2 antibody can be used for detection of SESTRIN2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
- SPECIFICITY: SESTRIN2 antibody is human, mouse and rat reactive. At least two isoforms of SESTRIN2 are known to exist; this antibody will detect both isoforms. SESTRIN2 antibody is predicted to not cross-react with SESTRIN1.
- POSITIVE CONTROL 1: Cat. No. 1405 - Mouse Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 1305 - Human Kidney Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 54 kDa
Observed: 53 kDa
- PURIFICATION: SESTRIN2 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: SESTRIN2 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: SESTRIN2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: SESN2
- ADDITIONAL NAMES: SESTRIN-2, SES2, SEST2, SESN2, HI95
- Protein Accession Number: NP_113647
- PROTEIN GI NUMBER: 13899299
- NCBI GENE ID NUMBER: 83667
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- SESTRIN2, also known as Hi95, is a member of the sestrin family of PA26-related proteins and is induced following DNA damage or oxidative stress (1). SESTRIN2 and the related protein SESTRIN1 interact with the NRF2 suppressor KEAP1, the autophagy substrate p62 and the ubiquitin ligase RBX1 to protect cells against oxidative stress by activating NRF2 and promoting the p62-dependent autophagic degradation of KEAP1 (2). SESTRIN2 also negatively regulates TOR signaling by activating AMP-activated protein kinase (AMPK) and TSC2 phosphorylation (3), thereby protecting cells against energetic stress-induced death (4).
- 1: Budanov AV, Shoshani T, Faerman A, et al. Identification of a novel stress-responsive gene Hi95 involved in regulation of cell viability. Oncogene 2002; 21:6017-31.
- 2: Bae SH, Sung SH, Oh SY, et al. Sestrins activate Nrf2 by promoting p62-dependent autophagic degradation of Keap1 and prevent oxidative liver damage. Cell Metab. 2013; 17:73-84.
- 3: Budanov AV and Karin M. p53 target genes sestrin1 and sestrin2 connect genotoxic stress and mTOR signaling. Mol. Cell 2008; 134:451-60.
- 4: Ben-Sahra I, Dirat B, Laurent K, et al. Sestrin2 integrates Akt and mTOR signaling to protect cells against energetic stress-induced death. Cell Death and Diff. 2013; 20:611-9.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 8023
- Size: 0.02 mg | 0.1 mg
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