Specifications
- HOST SPECIES: Goat
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Human, Mouse, Rat, Cow
- IMMUNOGEN: The immunogen for this antibody is: C-TRKENNLTEDNPN
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 90kDa band in Human Brain (Cerebellum) and Thymus lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 112kDa according to NP_001070671.1 and of 120kDa according to NP_065705.3. The 90kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
- SPECIFICITY: This antibody is expected to recognize both reported isoforms (NP_001070671.1; NP_065705.3)
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: SENP7
- ADDITIONAL NAMES: SENP7 , SUMO1/sentrin specific peptidase 7 , KIAA1707, MGC157730, SUMO1/sentrin specific protease 7, sentrin/SUMO-specific protease, sentrin/SUMO-specific protease 7
- Protein Accession Number: NP_001070671.1,NP_065705.3
- PROTEIN GI NUMBER: 116174736
- NCBI GENE ID NUMBER: 57337
Background
- 1: Gong, L., Millas, S., Maul, G.G. and Yeh, E.T Differential regulation of sentrinized proteins by a novel sentrin-specific protease. J. Biol. Chem. 275 (5), 3355-3359 (2000)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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