Specifications
- HOST SPECIES: Chicken
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (93%)
- IMMUNOGEN: SDHAF2 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human SDHAF2.
The immunogen is located within the first 50 amino acids of SDHAF2. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: SDHAF2 antibody can be used for detection of SDHAF2 by Western blot at 1 μg/mL.
Antibody validated: Western Blot in rat samples. All other applications and species not yet tested. - SPECIFICITY: SDHAF2 antibody is predicted to not cross-react with other SDHAF protein family members.
- POSITIVE CONTROL 1: Cat. No. 1464 - Rat Liver Tissue Lysate
Properties
- PURIFICATION: SDHAF2 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgY
- PHYSICAL STATE: Liquid
- BUFFER: SDHAF2 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: SDHAF2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: SDHAF2
- ADDITIONAL NAMES: SDHAF2 Antibody: PGL2, SDH5, C11orf79, PGL2, Succinate dehydrogenase assembly factor 2, mitochondrial, Succinate dehydrogenase subunit 5, SDH assembly factor 2
- Protein Accession Number: NP_060311
- PROTEIN GI NUMBER: 8923450
- NCBI GENE ID NUMBER: 54949
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- SDHAF2 Antibody: SDHAF2 (Succinate dehydrogenase complex assembly factor 2) encodes a mitochondrial protein needed for FAD cofactor attach to the SDH enzyme which plays a critical role in mitochondria. SDHAF2 is a tumor suppressor, but recent evidence suggests that somatic mutations of the SDHAF2 are unlikely to contribute to parathyroid tumor development in sporadic primary hyperparathyroidism.
- 1: Hao HX, Khalimonchuk O, Schraders M, et al. SDH5, a gene required for flavination of succinate dehydrogenase, is mutated in paraganglioma. Science 2009; 325:1139-42.
- 3: Kunst HP, Rutten MH, de Monnink JP, et al. SDHAF2 (PGL2-SDH5) and hereditary head and neck paraganglioma. Clin. Cancer Res. 2011; 17:247-54
- 4: Starker LF, Delgado-Verdugo A, Udelsman R, et al. Expression and somatic mutations of SDHAF2 (SDH5), a novel endocrine tumor suppressor gene in parathyroid tumors of primary hyperparathyroidism. Endocrine 2010; 38:397-401.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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