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Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Virus
- IMMUNOGEN: E.coli derived SARS-COV-2 Nucleoproteins Ser2-Ala419Gly335Ala Accession #QHD43423.2
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E
- APPLICATION NOTE: SARS-COV-2 Nucleocapsid Sandwich Elisa:
ELISA Capture: Recommended Concentration:0.5-2ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein AntibodyCAP)
ELISA Capture: Recommended Concentration:0.005-0.02ug/mL, Sample: SARS-CoV-2 Nucleocapsid Protein Antibody
Standard:Recommended Concentration: 156-10000pg/mL, Sample: Recombinant SARS-COV-2 Nucleocapsid Protein with His tag
- SPECIFICITY: SARS-COV-2
Properties
- PURIFICATION: Affinity purification
< 1.0 EU/ug of the protein by LAL method. - CLONALITY: Recombinant Monoclonal
- ISOTYPE: Rabbit IgG
- PHYSICAL STATE: Lyophilized
- BUFFER: Lyophilized from a 0.2 um filtered solution in PBS, pH7.4 with antibody at a final concentration of 1 mg/ml.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: 2°C - 8°C for one month, -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles
Additional Info
- NCBI OFFICIAL SYMBOL: N
- ADDITIONAL NAMES: SARS-CoV-2 Nucleocapsid Protein, SARS-CoV-2 NP, nucleocapsid protein [Severe acute respiratory syndrome coronavirus 2], novel coronavirus N Protein, novel coronavirus Nucleocapsid Protein, 2019-nCoV Nucleoprotein, 2019-nCoV N, 2019nCoV N, 2019-nCoV N Protein, 2019 peak N Protein, 2019-nCoV nucleocapsid protein.
- Protein Accession Number: QHD43423.2
- NCBI GENE ID NUMBER: 1489678
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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