SARS CoV-2 full length spike protein in LMNG detergent
CATALOG NUMBER: 21-815- Tested Applications: ELISA
- Size: 0.1 mg
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- SPECIES: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike Protein
- SOURCE SPECIES: HEK293 cells
- RECOMBINANT PROTEIN SEQUENCE: Full-length sequence (aa 1 – 1273),
furin cleavage site “RRAR” mutated to “GSAG”;
C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence - FUSION TAG: C-terminal Rho1D4
- TESTED APPLICATIONS: ELISA
- APPLICATION NOTE: This recombinant protein can be used for ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses. For research use only.
- PREDICTED MOLECULAR WEIGHT: 1286 amino acids (including Rho1D4 tag and linker); 142114 Da
- BIOLOGICAL ACTIVITY: not determined
Properties
- PURITY: >98% as determined by SDS-PAGE, see Fig. 1 A and B. PureCube Rho1D4 Agarose.
- PHYSICAL STATE: Liquid
- BUFFER: 20 mM Hepes pH 7.5; 150 mM NaCl, 0.01 % LMNG
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: –80°C. Avoid freeze-thaw cycles
Additional Info
- NCBI OFFICIAL SYMBOL: S
- ADDITIONAL NAMES: SARS-CoV-2 (COVID-19) Spike Recombinant Protein: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Surface Glycoprotein, Spike protein
- NCBI GENE ID NUMBER: 43740568
Background
- The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor. The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways: MSP nanodiscs, based on MSP proteins Detergent Mycelles, as you can see here Synthetic nanodiscs
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures.
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