Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Virus
- IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19) ORF3b antibody (9277) was raised against a peptide corresponding to 12 amino acids near carboxyl terminus of SARS-CoV-2 (COVID-19) ORF3b protein.
The immunogen is located within the last 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: WB: 0.1 μg/mL
Antibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested. - SPECIFICITY: ORF3b Antibody is predicted to not cross-react with other coronavirus family members.
- PREDICTED MOLECULAR WEIGHT: 17kD
Properties
- PURIFICATION: SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ORF3b
- ADDITIONAL NAMES: ORF3b protein, Acessary protein 3b, ns3b, Non-structural protein 3b, ORF3b
- Protein Accession Number: P0DTC3
- PROTEIN GI NUMBER: 1835921851
- NCBI GENE ID NUMBER: 43740569
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
- 1: Gorbalenya. bioRxiv: 2020.
- 2: Hui et al. Int J Infect Dis. 2020;91:264-266.
- 3: Konno et al. Cell Rep. 2020; 32:108185.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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