Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Virus
- HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV Nucleocapsid proteins: (100%)
- IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody (9099) was raised against a peptide corresponding to 17 amino acids near the center of SARS-CoV-2 (COVID-19) Nucleocapsid protein.
The immunogen is located within 230-280 amino acids of SARS-CoV-2 (COVID-19) Nucleocapsid protein. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: WB: 0.5-2 μg/mL
SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be used for the detection of SARS-CoV-2 (COVID-19) Nucleocapsid protein in ELISA. It will detect 4 ng of free peptide at 1 μg/mL. All other applications and species not yet tested.
Advanced Validation
- ISOFORMS: SARS-CoV-2 (COVID-19) Nucleocapsid has one isoform (419aa).
Properties
- PURIFICATION: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: SARS-CoV-2 (COVID-19) Nucleocapsid antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: N
- ADDITIONAL NAMES: SARS-CoV-2 (COVID-19) Nucleocapsid antibody: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Nucleocapsid protein, N protein, Nucleocapsid Phosphoprotein
- Protein Accession Number: QHD43423
- PROTEIN GI NUMBER: 1820552696
- NCBI GENE ID NUMBER: 43740575
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus. The disease is the cause of the 2019–20 coronavirus outbreak (1). SARS-CoV-2 is the seventh member of the enveloped, positive-stranded RNA viruses that are able to infect humans. The SARS-CoV-2 genome, like other coronaviruses, encodes for multiple structural and nonstructural proteins. The structural proteins include spike protein (S), envelope protein (E), membrane glycoprotein (M), nucleocapsid phosphoprotein (N), and the nonstructural proteins include open reading frame 1ab (ORF1ab), ORF3a, ORF6, ORF7a, ORF8, and ORF10 (2). Nucleocapsid (N) protein is the most abundant protein of coronavirus. It is also one of the major structural proteins and is involved in the transcription and replication of viral RNA, packaging of the encapsidated genome into virions (3), and interference with cell cycle processes of host cells (4). Moreover, in many coronaviruses, including SARS-CoV, the N protein has high immunogenic activity and is abundantly expressed during infection (5). It can be detected in various patient samples including nasopharyngeal aspirate, urine, and fecal. Both S and N proteins may be potential antigens for serodiagnosis of COVID-19, just as many diagnostic methods have been developed for diagnosing SARS based on S and/or N proteins (6).
- 1: Zhu et al. N Engl J Med. 2020 Feb 20;382(8):727-733.
- 2: Kiyotani et al. J Hum Genet. 2020 Jul;65(7):569-575.
- 3: Chang et al. J Biomed Sci. 2006 Jan;13(1):59-72.
- 4: Cui et al. J Virol. 2015 Sep;89(17):9029-43.
- 5: Chen et al. Eur J Clin Microbiol Infect Dis. 2005 Aug;24(8):549-53.
- 6: Emerg Infect Dis. 2004 Sep;10(9):1558-62.
Citations
- 1: Sun Y, Ge L, Rau MJ, Patton MD, Gallan AJ, Felix JC, Rui H. Sensitive and specific immunohistochemistry protocols for detection of SARS-CoV-2 nucleocapsid and spike proteins in formalin-fixed, paraffin-embedded COVID-19 patient tissues. ProtocolExchange, 16 July 2020; DOI:10.21203/rs.3.pex-1011/v1
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
SARS-CoV-2 Omicron BA.1 Variant Pseudovirus
CATALOG NUMBER: 95-201
SARS-CoV-2 WT Pseudovirus
CATALOG NUMBER: 95-200
SARS-CoV-2 (COVID-19) Spike Q493R G496S Q498R N501Y Y505H Antibody (Omicron)
CATALOG NUMBER: 9805