Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Virus
- HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV NSP14 (ExoN) protein: (79%).
- IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody (9185) was raised against a peptide corresponding to 14 amino acids near the center of SARS-CoV-2 (COVID-19) NSP14 (ExoN) protein.
The immunogen is located between 120-170 amino acids of SARS-CoV-2 (COVID-19) NSP14 (ExoN). - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC
- APPLICATION NOTE: IHC: 0.5 μg/mL .
Antibody validated: Immunohistochemistry in human samples. SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can detect 2 ng of free peptide at 1 μg/mL in ELISA. All other applications and species not yet tested
Properties
- PURIFICATION: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: SARS-CoV-2 (COVID-19) NSP14 (ExoN) Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: SARS-CoV-2 (COVID-19) NSP14 (ExoN) antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: ExoN
- ADDITIONAL NAMES: Proofreading exoribonuclease, Guanine-N7 methyltransferase, Non-structural protein 14, NSP14.
- Protein Accession Number: YP_009725309.1
- PROTEIN GI NUMBER: 1802476817
- NCBI GENE ID NUMBER: 43740578
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019–20 coronavirus outbreak (2). The structure of 2019-nCoV consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. NSP14 (ExoN) is an enzyme possessing two different activities: an exoribonuclease activity acting on both ssRNA and dsRNA in a 3' to 5' direction and a N7-guanine methyltransferase activity (3,4,5). It acts as a proofreading exoribonuclease for RNA replication, thereby lowering The sensitivity of the virus to RNA mutagens(6,7,8).
- 1: Gorbalenya. bioRxiv: 2020.
- 2: Hui et al. Int J Infect Dis. 2020;91:264-266.
- 3: Minskaia et. al. Proc. Natl. Acad. Sci. U.S.A 2006; 103:5108-5113
- 4: Bouvet et. al. PLoS Pathog. 2010; 6:E1000863-E1000863
- 5: Bouvet et. al. Proc. Natl. Acad. Sci. U.S.A. 2012;109:9372-9377
- 6: Denison et. al. RNA Biol. 2011;8:270-279
- 7: Smith et. al. PLoS Pathog. 2013;9:e1003565-e1003565
- 8: Agostini et. al. MBio 2018; 9:0-0
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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