Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: This RT25 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 145-173 amino acids from the C-terminal region of human RT25.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IHC-P, WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50 - PREDICTED MOLECULAR WEIGHT: 20 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: MRPS25
- ADDITIONAL NAMES: 28S ribosomal protein S25, mitochondrial, MRP-S25, S25mt, MRPS25, RPMS25
- Protein Accession Number: P82663
- PROTEIN GI NUMBER: 13633894
- NCBI GENE ID NUMBER: 64432
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4.
- 1: Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
- 2: Zhang, Z., et al. Genomics 81(5):468-480(2003)
- 3: Kenmochi, N., et al. Genomics 77 (1-2), 65-70 (2001) :
- 4: Cavdar Koc, E., et al. J. Biol. Chem. 276(22):19363-19374(2001)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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