Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
- IMMUNOGEN: This RPL12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 71-100 amino acids from the Central region of human RPL12.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
- PREDICTED MOLECULAR WEIGHT: 18 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: RPL12
- ADDITIONAL NAMES: 60S ribosomal protein L12, RPL12
- Protein Accession Number: P30050
- PROTEIN GI NUMBER: 266921
- NCBI GENE ID NUMBER: 6136
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Ribosomes, the organelles that catalyze protein synthesis,consist of a small 40S subunit and a large 60S subunit. Togetherthese subunits are composed of 4 RNA species and approximately 80structurally distinct proteins. This gene encodes a ribosomalprotein that is a component of the 60S subunit. The protein belongsto the L11P family of ribosomal proteins. It is located in thecytoplasm. The protein binds directly to the 26S rRNA. This gene isco-transcribed with the U65 snoRNA, which is located in its fourthintron. As is typical for genes encoding ribosomal proteins, thereare multiple processed pseudogenes of this gene dispersed throughthe genome.
- 1: Letra, A., et al. Am. J. Med. Genet.152A (7), 1701-1710 (2010) :
- 2: Wheeler, H.E., et al. PLoS Genet.(10), E1000685 (2009) :
- 3: Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
- 4: Olsen, J.V., et al. Cell 127(3):635-648(2006)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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