Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- IMMUNOGEN: The immunogen for this antibody is: C-KTAAVLKWNRE
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Not yet tested - our routinely used western blotting protocol does not allow detection of proteins as large as the calculated size of 374kDa according to NP_065965.3. Therefore we cannot recommend an optimal concentration and the product is investigative grade. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded human kidney shows rare staining of nucleoli across the kidney . Recommended concentration, 2.5ug/ml.
- POSITIVE CONTROL 1: Cat. No. 1305 - Human Kidney Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-401 - Human Kidney Tissue Slide
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: RNF213
- ADDITIONAL NAMES: RNF213, C17orf27, ring finger protein 213, chromosome 17 open reading frame 27, KIAA1554
- Protein Accession Number: NP_065965.4
- PROTEIN GI NUMBER: 66571327
- NCBI GENE ID NUMBER: 57674
Background
- 1: Zody MC et al DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage. Nature. 2006 Apr 20;440(7087):1045-9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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