Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine
- IMMUNOGEN: This RNF128 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-143 amino acids from the Central region of human RNF128.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: WB
- APPLICATION NOTE: For WB starting dilution is: 1:1000
- PREDICTED MOLECULAR WEIGHT: 47 kDa
Properties
- PURIFICATION: This antibody is purified through a protein A column, followed by peptide affinity purification.
- CLONALITY: Polyclonal
- ISOTYPE: Rabbit Ig
- PHYSICAL STATE: Liquid
- BUFFER: Supplied in PBS with 0.09% (W/V) sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: RNF128
- ADDITIONAL NAMES: E3 ubiquitin-protein ligase RNF128, 632-, Gene related to anergy in lymphocytes protein, GRAIL, RING finger protein 128, RNF128
- Protein Accession Number: Q8TEB7
- PROTEIN GI NUMBER: 74751443
- NCBI GENE ID NUMBER: 79589
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The protein encoded by this gene is a type I transmembraneprotein that localizes to the endocytic pathway. This proteincontains a RING zinc-finger motif and has been shown to possess E3ubiquitin ligase activity. Expression of this gene in retrovirallytransduced T cell hybridoma significantly inhibitsactivation-induced IL2 and IL4 cytokine production. Inducedexpression of this gene was observed in anergic CD4(+) T cells,which suggested a role in the induction of anergic phenotype.Alternatively spliced transcript variants encoding distinctisoforms have been reported.
- 1: Su, L.L., et al. J. Immunol. 183(1):438-444(2009)
- 2: Lin, J.T., et al. J. Immunol. 182(10):5919-5928(2009)
- 3: Lineberry, N., et al. J. Biol. Chem. 283(42):28497-28505(2008)
- 4: Egawa, S., et al. Am. J. Physiol. Gastrointest. Liver Physiol. 295 (1), G163-G169 (2008) :
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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