- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Mouse: (100%)
- IMMUNOGEN: RIPK1 antibody was raised against a 14 amino acid peptide near the carboxy terminus of human RIP1.
The immunogen is located within amino acids 600 - 650 of RIPK1.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P
- APPLICATION NOTE: RIPK1 antibody can be used for detection of RIP1 by immunohistochemistry at 5 - 10 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.
- PREDICTED MOLECULAR WEIGHT: Predicted: 74 kDa
- PURIFICATION: RIPK1 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: RIPK1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: RIPK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: RIPK1
- ADDITIONAL NAMES: RIPK1 Antibody: RIP, RIP1, RIP, Cell death protein RIP, RIP-1
- Protein Accession Number: NP_003795
- PROTEIN GI NUMBER: 57242761
- NCBI GENE ID NUMBER: 8737
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- RIP1 Antibody: RIP1 (Receptor Interacting Protein), also known as RIPK1, is a crucial 74 kD adaptor kinase in several of stress-induced signaling pathways and on the crossroad of a cell's decision to live or die. RIP1 contains an N-terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C-terminal region containing a death domain motif present in the Fas and TNFR1 intracellular domains. Full length RIP1 is important for signallling to NF-kappa-B, MAPKs and necrosis, whereas caspase-8 generates a C-terminal RIP1 cleavage fragment, promoting TNF-induced apoptosis. It is required for TNFRSF1A-mediated and TLR3-induced NF-kappa-B activation. RIP1-deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age.
- 1: Stanger BZ, Leder P, Lee TH, et al. RIP: a novel protein containing a death domain that interacts with Fas/APO-1 (CD95) in yeast and causes cell death. Cell 1995; 81:513-23.
- 2: Hsu H, Huang J, Shu HB, et al. TNF-dependent recruitment of the protein kinase RIP to the TNF receptor-1 signaling complex. Immunity 1996; 4:387-96.
- 3: Meylan E, Burns K, Hofmann K, et al. RIP1 is an essential mediator of Toll-like receptor 3-induced NF-kappa B activation. Nat. Immunol.; 2004; 5:503-7.
- 4: Festjens N, Vanden Bergh T, Cornelis S, et al. RIP1, a kinase on the crossroads of a cell's decision to live or die. Cell Death Differ. 2007;14:400-10.
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page.
- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 3291
- Size: 0.02 mg | 0.1 mg
- List Price: $89.00 – $395.00
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