- Trial Size
RICK Antibody
CATALOG NUMBER: 2183- Clonality: Polyclonal
- Tested Applications: E, ICC, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%)
- IMMUNOGEN: RICK antibody was raised against a 15 amino acid peptide near the carboxy terminus of human RICK.
The immunogen is located within the last 50 amino acids of RICK. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, ICC, IF, WB
- APPLICATION NOTE: RICK antibody can be used for detection of RICK by Western blot at 0.5 μg/mL. An approximately 60 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1204 - K562 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1212 - 3T3 Cell Lysate
- POSITIVE CONTROL 3: Cat. No. 17-004 - K-562 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 60 kDa
Observed: 58 kDa
Properties
- PURIFICATION: RICK Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: RICK Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/ml
- STORAGE CONDITIONS: RICK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: RIPK2
- ADDITIONAL NAMES: RICK Antibody: CCK, RICK, RIP2, CARD3, GIG30, CARDIAK, UNQ277/PRO314/PRO34092, CARD-containing interleukin-1 beta-converting enzyme-associated kinase, CARD-containing IL-1 beta ICE-kinase
- Protein Accession Number: O43353
- PROTEIN GI NUMBER: 20455217
- NCBI GENE ID NUMBER: 8767
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- RICK Antibody: Apoptosis is mediated by death domain (DD) and/or caspase recruitment domain (CARD) containing molecules and a caspase family of proteases. DD-containing serine/threonine kinase RIP regulates Fas-induced apoptosis. A novel CARD-containing serine/threonine kinase was recently identified and designated RICK/RIP2/CARDIAK for RIP-like interacting CLARP kinase, receptor interacting protein-2, and CARD-containing ICE associated kinase, respectively. RICK contains an N-terminal kinase catalytic domain and a C-terminal CARD domain. Overexpression of RICK induced apoptosis and activation of NF-κB and JNK. RICK interacts with members of the TRAF family, CLARP and caspase-1. Thus, RICK represents a novel kinase that regulates TNF and Fas induced-apoptosis and that is involved in the generation of proinflammatory cytokine IL-1β. The messenger RNA of RICK is expressed in multiple human tissues.
- 1: Inohara N, del Peso L, Koseki T, Chen S, Nunez G. RICK, a novel protein kinase containing a caspase recruitment domain, interacts with CLARP and regulates CD95-mediated apoptosis. J Biol Chem 1998;273:12296-300
- 2: McCarthy JV, Ni J, Dixit VM. RIP2 is a novel NF-κB-activating and cell death-inducing kinase. J Biol Chem 1998;273:16968-75
- 3: Thome M, Hofmann K, Burns K, Martinon F, Bodmer JL, Mattmann C, Tschopp J. Identification of CARDIAK, a RIP-like kinase that associates with caspase-1. Curr Biol 1998;8:885-8 (WD0300)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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