Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: Recombinant fusion protein containing a sequence corresponding to amino acids 181-340 of human RFC5 (NP_031396.1).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IHC, WB
- APPLICATION NOTE: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200 - POSITIVE CONTROL 1: HeLa
- POSITIVE CONTROL 2: U-251MG
- POSITIVE CONTROL 3: SKOV3
- POSITIVE CONTROL 4: MCF-7
- POSITIVE CONTROL 5: Mouse liver
- POSITIVE CONTROL 6: Mouse spleen
- PREDICTED MOLECULAR WEIGHT: Observed: 38kDa
Properties
- PURIFICATION: Affinity purification
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Store at -20°C. Avoid freeze / thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: RFC5
- ADDITIONAL NAMES: RFC5, MGC1155, RFC36
- NCBI GENE ID NUMBER: 5985
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 36 kD subunit. This subunit can interact with the C-terminal region of PCNA. It forms a core complex with the 38 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 9.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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