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Home / Cancer / Retinoblastoma / Rb Antibody [SPM353]
Retinoblastoma / Rb Antibody [SPM353]
Retinoblastoma / Rb Antibody [SPM353]

Retinoblastoma / Rb Antibody [SPM353]

CATALOG NUMBER: 33-784
  • Clonality: Monoclonal
  • Tested Applications: IHC-P, IP, WB
  • Host Species: Mouse
  • Species Reactivity: Human, Mouse
  • Conjugate: Unconjugated
  • Size: 100 ug

Specifications

  • HOST SPECIES: Mouse
  • SPECIES REACTIVITY: Human, Mouse
  • IMMUNOGEN: A human recombinant partial protein was used as the immunogen for this Retinoblastoma antibody.
  • CONJUGATE: Unconjugated
  • TESTED APPLICATIONS: IHC-P, IP, WB
  • APPLICATION NOTE: WB: 0.5-1 ug/ml
    IP: 1-2ug/500ug protein lysate
    IHC (FFPE): 1-2 ug/ml for 30 min at RT
    Titering of the Retinoblastoma antibody may be required for optimal performance.

Properties

  • PURIFICATION: Protein G affinity chromatography
  • CLONALITY: Monoclonal
  • ISOTYPE: IgG1, kappa
  • PHYSICAL STATE: Liquid
  • BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
  • CONCENTRATION: 0.2 mg/mL
  • STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.

Additional Info

  • NCBI OFFICIAL SYMBOL: RB1
  • ADDITIONAL NAMES: Retinoblastoma-associated protein, p105-Rb, pRb, Rb, pp110, RB1
  • NCBI GENE ID NUMBER: 5925
  • USER NOTE: Optimal dilutions for each application to be determined by the researcher

Background

  • Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Disclaimer

  • FOR RESEARCH USE ONLY
    For additional information, visit ProSci's Terms & Conditions Page.
  • Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER: 33-784

  • Size: 100 ug
  • List Price: $535.00
Datasheet
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