Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Mouse
- IMMUNOGEN: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mResistin (murine Resistin).
- CONJUGATE: Biotin
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: ELISA:
Sandwich:
To detect mResistin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine Resistin (XP-5260) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mResistin.
Western Blot:
To detect mResistin by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mResistin is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-mResistin specific antibody was purified by affinity chromatography and then biotinylated.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Resistin antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: Retn
- ADDITIONAL NAMES: ADSF, Rstn, Xcp4, Fizz3, Resistin, Adipose tissue-specific secretory factor, ADSF
- Protein Accession Number: Q99P87
- PROTEIN GI NUMBER: 17380301
- NCBI GENE ID NUMBER: 57264
- USER NOTE: Centrifuge vial prior to opening.
Background
- Resistin, also known as Adipocyte Secreted Factor (ADSF) and FIZZ3, is a cytokine that is specifically secreted by adipocytes and seems to suppress the ability of insulin to stimulate glucose uptake into adipose cells. While the function of resistin is not well understood, it appears to have key paracrine roles and has a suggested role in type 2 diabetes.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
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