Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Recombinant protein was used as the immunogen for the Calponin antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: Flow, IF, IHC-P
- APPLICATION NOTE: Flow Cytometry: 0.5-1ug/10^6 cells
Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Optimal dilution of the Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
Properties
- PURIFICATION: Protein A affinity chromatography
- CLONALITY: Recombinant Monoclonal
- ISOTYPE: IgG, k
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CNN1
- ADDITIONAL NAMES: CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp
- NCBI GENE ID NUMBER: 1264
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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