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Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: A recombinant full length human protein was used as the immunogen for the CAD antibody.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: IF, IHC-P
- APPLICATION NOTE: Immunofluorescence: 0.5-1 ug/ml
Immunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)
Titering of the CAD antibody may be required for optimal performance.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min.
Properties
- PURIFICATION: Protein A affinity chromatography
- CLONALITY: Recombinant Monoclonal
- ISOTYPE: IgG, k
- PHYSICAL STATE: Liquid
- BUFFER: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
- CONCENTRATION: 0.2 mg/mL
- STORAGE CONDITIONS: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: CALD1
- ADDITIONAL NAMES: Caldesmon, CDM, CALD1, CAD, CDM
- NCBI GENE ID NUMBER: 800
- USER NOTE: Optimal dilutions for each application to be determined by the researcher
Background
- Caldesmon is an actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). [UniProt]
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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