Specifications
- HOST SPECIES: Goat
- SPECIES REACTIVITY: Human
- HOMOLOGY: Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
- IMMUNOGEN: The immunogen for this antibody is: C-DRYSGGNYRDNYDN
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, WB
- APPLICATION NOTE: Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx.17kDa band observed in lysates of cell line HepG2 (calculated MW of 17.2kDa according to NP_006734.1). Recommended concentration: 0.01-0.03ug/ml.An additional band of unknown identity was also consistently observed at 48kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Properties
- PURIFICATION: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
- CONCENTRATION: 500 ug/mL
- STORAGE CONDITIONS: Aliquot and store at -20°C. Minimize freezing and thawing.
Additional Info
- NCBI OFFICIAL SYMBOL: RBM3
- ADDITIONAL NAMES: RBM3, RNA binding motif (RNP1, RRM) protein 3, IS1-RNPL, RNPL, OTTHUMP00000025800, OTTHUMP00000025802, RNA binding motif protein 3
- Protein Accession Number: NP_006734.1
- PROTEIN GI NUMBER: 5803137
- NCBI GENE ID NUMBER: 5935
Background
- 1: Sureban SM, Ramalingam S, Natarajan G, May R, Subramaniam D, Bishnupuri KS, Morrison AR, Dieckgraefe BK, Brackett DJ, Postier RG, Houchen CW, Anant S. Translation regulatory factor RBM3 is a proto-oncogene that prevents mitotic catastrophe. Oncogene. 2008 Jul 31;27(33):4544-56.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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