- Trial Size
RAP80 Antibody
CATALOG NUMBER: 4327- Clonality: Polyclonal
- Tested Applications: E, IF, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Pig: (100%), Rat: (88%)
- IMMUNOGEN: RAP80 antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human RAP80.
The immunogen is located within amino acids 30 - 80 of RAP80. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: RAP80 antibody can be used for detection of RAP80 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1210 - HEK293 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide
Properties
- PURIFICATION: RAP80 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: RAP80 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: RAP80 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: UIMC1
- ADDITIONAL NAMES: RAP80 Antibody: RAP80, X2HRIP110, RAP80, RXRIP110, BRCA1-A complex subunit RAP80, Receptor-associated protein 80
- Protein Accession Number: EAW85043
- PROTEIN GI NUMBER: 119605449
- NCBI GENE ID NUMBER: 51720
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- RAP80 Antibody: RAP80 was initially identified as zinc-finger containing nuclear protein that is highly expressed in testis and interacts with the retinoid-related testis-associated receptor (RTR). Later experiments revealed that RAP80 is recruited by the Coiled-coil domain 98 (CCDC98) protein to the breast cancer-1 protein BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both RAP80 and CCDC98 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either RAP80 or CCDC98 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that RAP80 and CCDC98 control only part of the DNA damage response role of BRCA1. At least four isoforms of RAP80 are known to exist.
- 1: Yan Z, Kim YS, and Jetten AM. RAP80, a novel nuclear protein that interacts with the retinoid-related testis-associated receptor. J. Biol. Chem.2002; 277:32379-88.
- 2: Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science2007; 316:1194-1198.
- 3: Kim H, Huang J, and Chen J. CCDC98 is a BRCA1-BRCT domain-binding protein involved in the DNA damage response. Nat. Struct. Mol. Biol.2007; 14:710-5.
- 4: Liu Z, Wu J, and Yu X. CCDC98 targets BRCA1 to DNA damage sites. Nat. Struct. Mol. Biol.2007; 14:716-20.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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