- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Rat: (95%), Mouse: (95%)
- IMMUNOGEN: Rabbit polyclonal PTRF antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PTRF.
The immunogen is located within amino acids 270 - 320 of PTRF.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: PTRF antibody can be used for detection of PTRF by Western blot at 0.5 - 1 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: At least three isoforms of PTRF are known to exist; this antibody detects all three isoforms. It is predicted to not cross-react with other members of the cavin family.
- POSITIVE CONTROL 1: Cat. No. 1306 - Human Spleen Tissue Lysate
- POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide
- PREDICTED MOLECULAR WEIGHT: 43 kDa
- PURIFICATION: PTRF Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PTRF Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PTRF antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
- NCBI OFFICIAL SYMBOL: PTRF
- ADDITIONAL NAMES: PTRF Antibody: CGL4, CAVIN, CAVIN1, FKSG13, cavin-1, Polymerase I and transcript release factor, Cavin-1
- Protein Accession Number: NP_036364
- PROTEIN GI NUMBER: 42734430
- NCBI GENE ID NUMBER: 284119
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- PTRF Antibody: The polymerase I and transcript release factor (PTRF) enables the dissociation of paused ternary polymerase I transcription complexes from the 3' end of pre-rRNA transcripts, regulating rRNA transcription by promoting the dissociation of transcription complexes and the re-initiation of polymerase I on nascent rRNA transcripts. PTRF also localizes to caveolae at the plasma membrane and is thought to play a critical role in the formation of caveolae and the stabilization of caveolins, translocating from caveolae to the cytoplasm after insulin stimulation. PTRF is also thought to modify lipid metabolism and insulin-regulated gene expression. Mutations in this gene result in a disorder characterized by generalized lipodystrophy and muscular dystrophy.
- 1: Jansa P, Mason SW, Hoffmann-Rohrer U, et al. Cloning and functional characterization of PTRF, a novel protein which induces dissociation of paused ternary transcription complexes. EMBO J. 1998; 17:2855-64.
- 2: Vinten J, Johnsen AH, Roepstorff P, et al. Identification of a major protein on the cytosolic face of caveolae. Biochim. Biophys. Acta 2005; 1717:34-40.
- 3: Aboulaich N, Ortegren U, Vener AV, et al. Association and insulin regulated translocation of hormone-sensitive lipase with PTRF. Biochem. Biophys. Res. Commun. 2006; 350:657-61.
- 4: Aboulaich N, Chui PC, Asara JM, et al. Polymerase I and transcript release factor regulates lipolysis via a phosphorylation-dependent mechanism. Diabetes 2011; 60:757-65.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 7267
- Size: 0.02 mg | 0.1 mg
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