- Trial Size
PROM1 Antibody
CATALOG NUMBER: 7783- Clonality: Polyclonal
- Tested Applications: ELISA, ICC, IF, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: PROM1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PROM1.
The immunogen is located within the last 50 amino acids of PROM1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, ICC, IF, WB
- APPLICATION NOTE: PROM1 antibody can be used for detection of PROM1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 2.5 μg/mL. For Immunoflorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. - SPECIFICITY: PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-005 - Jurkat Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 95 kDa
Observed: 97 kDa
Properties
- PURIFICATION: PROM1 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PROM1 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PROM1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PROM1
- ADDITIONAL NAMES: PROM1 Antibody: RP41, AC133, CD133, MCDR2, STGD4, CORD12, PROML1, MSTP061, Prominin-1, Antigen AC133
- Protein Accession Number: NP_006008
- PROTEIN GI NUMBER: 5174387
- NCBI GENE ID NUMBER: 8842
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PROM1 is a pentaspan transmembrane glycoprotein that localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation (1). Mutations in this gene have been shown to result in retinitis pigmentosa (2). Expression of this gene is also associated with several types of cancer (3).
- 1: Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997; 90:5002-12.
- 2: Zhang Q, Zulfiqar F, Xiao X, et al. Severe retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the PROM1 gene. Hum. Genet. 2007; 122:293-9.
- 3: Tabu K, Bizen N, Taga T, et al. Gene regulation of Prominin-1 (CD133) in normal and cancerous tissues. Adv. Exp. Med. Biol. 2013; 777:73-85.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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