PRKCZ Antibody
CATALOG NUMBER: 29-814- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 100 ul
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRKCZ.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: PRKCZ antibody can be used for detection of PRKCZ by ELISA at 1:62500. PRKCZ antibody can be used for detection of PRKCZ by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- POSITIVE CONTROL 1: Cat. No. 1205 - Jurkat Cell Lysate
- PREDICTED MOLECULAR WEIGHT: 45 kDa, 47 kDa, 68 kDa
Properties
- PURIFICATION: Antibody is purified by peptide affinity chromatography method.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Liquid
- BUFFER: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: For short periods of storage (days) store at 4°C. For longer periods of storage, store PRKCZ antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: PRKCZ
- ADDITIONAL NAMES: PRKCZ, PKC2, PKC-ZETA
- Protein Accession Number: NP_001028753
- PROTEIN GI NUMBER: 75709226
- NCBI GENE ID NUMBER: 5590
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC.Protein kinase C (PKC) zeta is a member of the PKC family of serine/threonine kinases which are involved in a variety of cellular processes such as proliferation, differentiation and secretion. Unlike the classical PKC isoenzymes which are calcium-dependent, PKC zeta exhibits a kinase activity which is independent of calcium and diacylglycerol but not of phosphatidylserine. Furthermore, it is insensitive to typical PKC inhibitors and cannot be activated by phorbol ester. Unlike the classical PKC isoenzymes, it has only a single zinc finger module. These structural and biochemical properties indicate that the zeta subspecies is related to, but distinct from other isoenzymes of PKC. Alternative splicing results in multiple transcript variants encoding different isoforms.
- 1: Xie, Z., (2006) J. Biol. Chem. 281 (10), 6366-6375.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
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