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PRAK Antibody [7H10B4]
CATALOG NUMBER: 32-205- Clonality: Monoclonal
- Tested Applications: E, IHC, WB
- Host Species: Mouse
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mL
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- HOST SPECIES: Mouse
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC, WB
- APPLICATION NOTE: Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Properties
- CLONALITY: Monoclonal
- ISOTYPE: IgG1
- BUFFER: Ascitic fluid containing 0.03% sodium azide.
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: PRAK monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: MAPKAPK5
- ADDITIONAL NAMES: PRAKMAPK-activated protein kinase 5, MK5, MK-5, PRAK, MAPKAP-K5
- Protein Accession Number: Q8IW41
- PROTEIN GI NUMBER: 52000829
- NCBI GENE ID NUMBER: 8550
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
- 1: Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97(8):629-40.
- 2: Wijtten PJ. Prak R. Lemme A. et al. Br Poult Sci. 2004, Aug, 45(4):504-11.
- 3: New L. Jiang Y. Han J. Mol Biol Cell. 2003, Jun, 14(6):2603-16. Epub 2003 Mar 20.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
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