- Trial Size
PPAPDC1B Antibody
CATALOG NUMBER: 5515- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
Powered by BiozSpecifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- IMMUNOGEN: PPAPDC1B antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human PPAPDC1B.
The immunogen is located within amino acids 30 - 80 of PPAPDC1B. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE: PPAPDC1B antibody can be used for detection of PPAPDC1B by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in mouse samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1287 - EL4 Cell Lysate
Properties
- PURIFICATION: PPAPDC1B Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PPAPDC1B Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PPAPDC1B antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PPAPDC1B
- ADDITIONAL NAMES: PPAPDC1B Antibody: DPPL1, HTPAP, DPPL1, Phosphatidate phosphatase PPAPDC1B, Phosphatidic acid phosphatase type 2 domain-containing protein 1B
- Protein Accession Number: NP_001096029
- PROTEIN GI NUMBER: 156523237
- NCBI GENE ID NUMBER: 84513
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PPAPDC1B Antibody: Phosphatidate phosphatase (PAP) plays important role in lipid-signaling metabolism in eukaryotic cells. Two distinct types of PAP (PAP1 and PAP2) activity have been distinguished by their subcellular localization and differential sensitivity to N-ethylmaleimide (NEM) and Mg2+. A yeast diacylglycerol pyrophosphate (DGPP) phosphatase (DPP1) and mammalian DGPP phosphatase (PAP2) have been identified as Mg2+-independent and NEM-insensitive membrane-associated. PPAPDC1A (also known as DPPL2) and PPAPDC1B (DPPL1) form a novel type of Mg2+-independent and NEM-sensitive mammalian phosphatidate phosphatase showing broad substrate specificity. Knockdown experiments indicated that this protein is involved with multiple cell signaling pathways, including the JAK-Stat3, MAP kinase, and PKC pathways. PPAPDC1B may also potentiate the estrogen receptor pathway by down-regulating DUSP22.
- 1: Takeuchi M, Harigai M, Momohara S, et al. Cloning and characterization of DPPL1 and DPPL2, representatives of a novel type of mammalian phosphatidate phosphatase. Gene2007; 399:174-80.
- 2: Bernard-Pierrot I, Gruel N, Stransky N, et al. Characterization of the recurrent 8p11-12 amplicon identifies PPAPDC1B, a phosphatase protein, as a new therapeutic target in breast cancer. Cancer Res.2008; 68:7165-75.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
Note: Online orders only accepted for shipment within the USA. Click here for the list of international distributors.
New & Featured Products
![HIV-1 p24 Antibody [10A2] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-10A2_WB_PM-7371.gif){kind=small}
HIV-1 p24 Antibody [10A2] (biotin)
CATALOG NUMBER: PM-7371-biotin
![HIV-1 p24 Antibody [9D2F6] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-9D2F6_WB_PM-7369.gif){kind=small}
HIV-1 p24 Antibody [9D2F6] (biotin)
CATALOG NUMBER: PM-7369-biotin
![HIV-1 p24 Antibody [8D7E11] (biotin)](https://www.prosci-inc.com/wp-content/uploads/2023/01/HIV-1-p24-Antibody-8D7E11_WB_PM-7367.gif){kind=small}
HIV-1 p24 Antibody [8D7E11] (biotin)
CATALOG NUMBER: PM-7367-biotin