- Trial Size
PMEPA1 Antibody
CATALOG NUMBER: 8261- Clonality: Polyclonal
- Tested Applications: E, IHC-P, WB
- Host Species: Rabbit
- Species Reactivity: Human, Mouse, Rat
- Conjugate: Unconjugated
- Size: 0.02 mg | 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- IMMUNOGEN: PMEPA1 antibody was raised against a 16 amino acid peptide near the center of human PMEPA1.
The immunogen is located within amino acids 160 - 210 of PMEPA1. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IHC-P, WB
- APPLICATION NOTE: PMEPA1 antibody can be used for detection of PMEPA1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. - SPECIFICITY: PMEPA1 antibody is human, mouse and rat reactive. At least four isoforms are known to exist; this antibody will detect all of the isoforms.
- POSITIVE CONTROL 1: Cat. No. 1203 - A549 Cell Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 26, 28, 32 kDa
Observed: 27 kDa
Properties
- PURIFICATION: PMEPA1 antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PMEPA1 antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PMEPA1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Additional Info
- NCBI OFFICIAL SYMBOL: PMEPA1
- ADDITIONAL NAMES: Prostate transmembrane protein androgen induced 1, STAG1, TMEPAI
- Protein Accession Number: NP_064567
- PROTEIN GI NUMBER: 21361841
- NCBI GENE ID NUMBER: 56937
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- The prostate transmembrane protein, androgen induced 1 (PMEPA1) protein is a transmembrane protein that contains a Smad interacting motif (SIM) (1,2). Expression of this gene is induced by androgens and transforming growth factor beta, and the encoded protein suppresses the androgen receptor and transforming growth factor beta signaling pathways though interactions with Smad proteins (3). Overexpression of this gene may play a role in multiple types of cancer (2,4).
- 1: Xu LL, Shanmugam N, Segawa T, et al. A novel androgen-regulated gene, PMEPA1, located on chromosome 20q!3 exhibits high level expression in prostate. Genomics 2000; 66:257-63.
- 2: Rae FK, Hooper JD, Nicol DL, et al. Characterization of a novel gene, STAG1/PMEPA1, upregulated in renal cell carcinoma and other solid tumors. Mol. Carcinog. 2001; 32:44-53.
- 3: Watanabe Y, Itoh S, Goto T, et al. TMEPAI, a transmembrane TGF-beta-inducible protein, sequesters Smad proteins from active participation in TGF-beta signaling. Mol. Cell 2010; 37:123-34.
- 4: Vo Nguyen TT, Watanabe Y, Shiba A, et al. TMEPAI/PMEPA1 enhances tumorigenic activities in lung cancer cells. Cancer Sci. 2014; 105:334-41.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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