- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (100%), Rat: (94%)
- IMMUNOGEN: PIG-Y antibody was raised against a 16 amino acid synthetic peptide near the center of human PIG-Y.
The immunogen is located within amino acids 50 - 100 of PIG-Y.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: PIG-Y antibody can be used for detection of PIG-Y by Western blot at 1 - 2 μg/mL. Despite its predicted molecular weight, PIG-Y often migrates at 28-30 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
- SPECIFICITY: This antibody only detects isoform 1 of PIG-Y.
- POSITIVE CONTROL 2: Cat. No. 10-901 - Human Spleen Tissue Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 8 kDa
Observed: 16 kDa
- PURIFICATION: PIG-Y Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PIG-Y Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PIG-Y antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: PIGY
- ADDITIONAL NAMES: PIG-Y Antibody: PIG-Y, Phosphatidylinositol N-acetylglucosaminyltransferase subunit Y, Phosphatidylinositol-glycan biosynthesis class Y protein, PIG-Y
- Protein Accession Number: NP_116295
- PROTEIN GI NUMBER: 14249680
- NCBI GENE ID NUMBER: 84992
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- PIG-Y Antibody: Glycosylphosphatidylinositol (GPI) lipid anchoring is an important post-translational modification of proteins that takes place in the endoplasmic reticulum. The synthesis of GPI is initiated by GPI-N-acetylglucosaminyltransferase (GPI-GnT), a complex of proteins including PIG-A, PIG-H, PIG-C, GPI1, and DPM2. PIG-Y, the mammalian homolog to yeast Eri1p, is also thought to be involved in the biosynthesis of GPI. The PIG-Y gene encodes two proteins, one of which arises from leaky scanning of the mRNA.
- 1: Eisenhaber B, Maurer-Stroh S, Novatchkova M, et al. Enzymes and auxiliary factors for GPI lipid anchor biosynthesis and post-translational transfer to proteins. Bioessays2003; 25:367-85.
- 2: Watanabe R, Murakami Y, Marmor MD, et al. Initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-P and is regulated by DPM2. EMBO J.2000; 19:4402-11.
- 3: Murakami Y, Siripanyaphinoyo U, Hong Y, et al. The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component. Mol. Biol. Cell2005; 16:5236-46.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 4955
- Size: 0.02 mg | 0.1 mg
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