- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (85%)
- IMMUNOGEN: Anti-PHAP I antibody (3151) was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human PHAP I .
The immunogen is located within the last 50 amino acids of PHAP I.
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: E, IF, IHC-P, WB
- APPLICATION NOTE: WB: 1 μg/mL; IHC: 2 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in mouse samples; Immunofluorescence in mouse samples. All other applications and species not yet tested.
- SPECIFICITY: This polyclonal antibody has no cross-reaction to PHAP I2a and PHAP III.
- POSITIVE CONTROL 1: Cat. No. 1207 - Raji Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 1416 - Mouse Testis Tissue Lysate
- POSITIVE CONTROL 3: Cat. No. 1476 - Rat Testis Tissue Lysate
- POSITIVE CONTROL 4: Cat. No. 1408 - Mouse Small Intestine Tissue Lysate
- PREDICTED MOLECULAR WEIGHT: Predicted: 29kD
Observed: 29 kD
- PURIFICATION: PHAP I Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PHAP I Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PHAP I antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
- NCBI OFFICIAL SYMBOL: ANP32A
- ADDITIONAL NAMES: PHAP I Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32
- Protein Accession Number: P39687
- PROTEIN GI NUMBER: 730318
- NCBI GENE ID NUMBER: 8125
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
- PHAP I Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.
- 1: Jiang et al. Science. 2003;299(5604):223-6.
- 2: Nicholson and Thornberry. Science. 2003 10;299(5604):214-5.
- 1: Schafer et al. Enhanced sensitivity to cytochrome c-induced apoptosis mediated by PHAPI in breast cancer cells. Cancer Res. 2006;66(4):2210-8. PMID: 16489023
- 2: Kurokawa et al. A network of substrates of the E3 ubiquitin ligases MDM2 and HUWE1 control apoptosis independently of p53. Sci Signal. 2013;6(274):ra32. PMID: 23652204
- 3: Parrish. Regulation of Apoptosis Following Mitochondrial Cytochrome c Release. Department of Pharmacology and Molecular Cancer Biology, Duke University. PhD Thesis, 2010.
- FOR RESEARCH USE ONLY
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- Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
CATALOG NUMBER: 3151
- Size: 0.02 mg | 0.1 mg
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