Independent Antibody Validation in Cell lines (Figure 2) shows similar PHAP I expression profile in human, mouse and rat cell lines detected by two independent anti-PHAP I antibodies that recognize different epitopes, 3145 against C-terminus domain and 3151 against C-terminus different domain. PHAP I proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies.

KD validation (Figure 6): Anti-PHAP I antibody (3151) specificity was further verified by PHAP I specific knockdown. PHAP I signal in T47D cells transfected with PHAP I siRNAs was disrupted in comparison with that in cells transfected with control siRNAs.

Regulated expression validation (Figure 7, 8, 9): PHAP I expression detected by anit-PHAP I antibodies (3151) was up-regulated in breast cancer tissues of patients (Figure 7) and the breast cancer cell line (Figure 8) as well as in the Atxn1 KO mice (Figure 9).