PF-4 Antibody
CATALOG NUMBER: XP-5256- Clonality: Polyclonal
- Tested Applications: ELISA, WB
- Host Species: Rabbit
- Species Reactivity: Human
- Conjugate: Unconjugated
- Size: 0.1 mg
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- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human
- IMMUNOGEN: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hPF-4 (human Platelet Factor-4).
- CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, WB
- APPLICATION NOTE:
ELISA:
To detect hPF-4 by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.
Sandwich:
To detect hPF-4 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human PF-4 (XP-5256Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hPF-4.
Western Blot:
To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Properties
- PURIFICATION: Anti-hPF-4 specific antibody was purified by affinity chromatography employing immobilized hPF-4 matrix.
- CLONALITY: Polyclonal
- PHYSICAL STATE: Lyophilized
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: PF-4 antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.
Additional Info
- NCBI OFFICIAL SYMBOL: PF4
- ADDITIONAL NAMES: PF-4, CXCL4, SCYB4, Platelet factor 4, C-X-C motif chemokine 4, PF-4
- Protein Accession Number: P02776
- PROTEIN GI NUMBER: 130304
- NCBI GENE ID NUMBER: 5196
- USER NOTE: Centrifuge vial prior to opening.
Background
- Platelet factor 4 (PF-4) is a 70 amino acid protein that is released from the alpha granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF-4 probably has a role in inflammation and wound repair.
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: This product is for research use only.
Shipping Info
$65 Standard Overnight flat rate for as many products shipped within the United States.
$35 Dry Ice Shipment fee may be required on some items.
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