Specifications
- HOST SPECIES: Rabbit
- SPECIES REACTIVITY: Human, Mouse, Rat
- HOMOLOGY: Predicted species reactivity based on immunogen sequence: monkey (100%), horse (100%), gorilla (100%), chimpanzee (100%)
- IMMUNOGEN: Anti-PEX10 antibody (9671) was raised against a peptide corresponding to 10 amino acids near the carboxyl terminus of human PEX10.
The immunogen is located within last 50 amino acids of PEX10. - CONJUGATE: Unconjugated
- TESTED APPLICATIONS: ELISA, IF, WB
- APPLICATION NOTE: WB: 4 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunofluorescence in human, mouse and rat samples. All other applications and species not yet tested. - POSITIVE CONTROL 1: Cat. No. 1203 – A549 Cell Lysate
- POSITIVE CONTROL 2: Cat. No. 17-003 – A549 Cell Slide
- PREDICTED MOLECULAR WEIGHT: Predicted: 37kD
Observed: 37 kD
Advanced Validation
- ISOFORMS: Human PEX10 has 2 isoforms, including isoform 1 (326aa, 37.1kD) and isoform 2 (346aa, 39.2kD). Mouse PEX10 has 1 isoform (324aa, 37.2kD). 9671 can detect PEX10 in human and mouse.
Properties
- PURIFICATION: PEX10 Antibody is affinity chromatography purified via peptide column.
- CLONALITY: Polyclonal
- ISOTYPE: IgG
- PHYSICAL STATE: Liquid
- BUFFER: PEX10 Antibody is supplied in PBS containing 0.02% sodium azide.
- CONCENTRATION: 1 mg/mL
- STORAGE CONDITIONS: PEX10 antibody can be stored at 4 °C for three months and -20 °C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
- NCBI OFFICIAL SYMBOL: PEX10
- ADDITIONAL NAMES: PEX10 Antibody: Peroxin-10, Peroxisomal biogenesis factor 10, Peroxisome assembly protein 10, RING finger protein 69, RNF69
- Protein Accession Number: O60683
- PROTEIN GI NUMBER: 3914299
- NCBI GENE ID NUMBER: 5192
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- PEX10 is implicated in the biogenesis of peroxisomes. Mutations in this gene result in phenotypes within the Zellweger spectrum of peroxisomal biogenesis disorders, ranging from neonatal adrenoleukodystrophy to Zellweger syndrome.
- 1: Warren D.S. et al. Am. J. Hum. Genet. 63:347-359(1998)
- 2: Okumoto K. et al. Hum. Mol. Genet. 7:1399-1405(1998)
- 3: Ota T. et al. Nat. Genet. 36:40-45(2004)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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