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Perforin Antibody [CB5.4] (FITC)
CATALOG NUMBER: XA-1004F- Clonality: Monoclonal
- Tested Applications: ICC, IHC, IP, WB
- Host Species: Rat
- Species Reactivity: Mouse
- Conjugate: FITC
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- HOST SPECIES: Rat
- SPECIES REACTIVITY: Mouse
- IMMUNOGEN: Perforin (monoclonal CB5.4) antibody was raised against a recombinant mouse perforin, amino acids 98-534.
- CONJUGATE: FITC
- TESTED APPLICATIONS: ICC, IHC, IP, WB
- APPLICATION NOTE: Perforin antibody can be used for detection of mouse perforin (region aa 402-534) by immunocytochemistry, immunohistochemistry (frozen sections, paraffin sections), immunoprecipitation and western blot. Method: A mouse T cell clone was plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washings in PBS, 0.1% BSA, slides were incubated with 5 μg/mL of CB5.4 antibody, washed in PBS, incubated in anti-rat IgG-HRP, washed in PBS, and developed in appropriate substrate solution (DAB or AEC). Method: Cell extracts from the T cell clone B6.1 (2x106) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the CB5.4 antibody at 1 μg/mL. Proteins were visualized using a peroxidase-conjugated antibody to rat IgG and a chemiluminescence detection system. This product is for research use only.
Properties
- PURIFICATION: Perforin (monoclonal CB5.4) antibody was purified to ≥95% in SDS-PAGE.
- CLONALITY: Monoclonal
- ISOTYPE: IgG2a
- PHYSICAL STATE: Liquid
- BUFFER: PBS containing 0.02% sodium azide
- CONCENTRATION: batch dependent
- STORAGE CONDITIONS: Perforin antibody can be stored at 4°C, stable for one year.
Additional Info
- NCBI GENE ID NUMBER: 18646
- USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background
- 1: The calcium-binding protein calreticulin is a major constituent of lytic granules in cytolytic T lymphocytes: M. Dupuis, et al.; J. Exp. Med. 177, 1 (1993).
- 2: Resistance of CTL to perforin-mediated lysis. Evidence for a lymphocyte membrane protein interacting with perforin: C. Muller and J. Tschopp; J. Immunol. 153, 2470 (1994).
- 3: Cubilin, a binding partner for galectin-3 in the murine utero-placental complex: S. Crider-Pirkle, et al.; J. Biol. Chem. 277, 15904 (2002).
- 4: Fas-Fas Ligand Interactions Are Essential for the Binding to and Killing of Activated Macrophages by {gamma}{delta} T Cells: J.E. Dalton, et al.; J. Immunol. 173, 3660 (2004)
Disclaimer
- FOR RESEARCH USE ONLY
For additional information, visit ProSci's Terms & Conditions Page. - Disclaimer: Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Shipping Info
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